Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Gibco™ EBSS, no calcium, no magnesium, no phenol red
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Earle's Balanced Salt Solution (EBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Dry-blend Buffered Packs
Our quality control ensures that every pack of dry-blend buffers yields consistent solutions without weighing or pH adjustment. Simply empty contents of one foil envelope pack into a beaker, add appropriate solvents, and stir to dissolve.
| Physical Form | Powder |
|---|---|
| Product Line | BupH |
Gibco™ PBS Tablets
Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ xMAP Sheath Fluid Plus
xMAP Sheath Fluid Plus is the delivery medium that carries the sample to the optics component of Luminex xMAP technology-based instruments.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ PBS (Phosphate-Buffered Saline) Tablets
Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cellular applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Gibco™ DPBS (10X), calcium, magnesium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Gibco™ PB-MAX™ Karyotyping Medium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Fully supplemented medium developed for short-term culture of peripheral blood lymphocytes for cytogenetic studies and in vitro diagnostic procedures
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Includes | L-Glutamine |
|---|---|
| Color | Red |
| Physical Form | Liquid |
| Chemical Name or Material | Karyotyping Medium |
| Recommended Storage | Storage conditions: -5 to -20°C. Protect from light. Shipping conditions: Frozen Shelf life: 12 months from date of manufacture |
| Concentration | 1 X |
| For Use With (Application) | Cell Viability; Proliferation & Function; Clinical & Translational Research; Mammalian Cell Culture |
| Sterility | Sterile-filtered |
| Product Line | PB-MAX |
Gibco™ CTS™ DPBS, calcium, magnesium, bag format
Gibco Cell Therapy Systems (CTS) DPBS is the classical Dulbecco's phosphate buffered saline, which is a balanced salt solution used for a variety of cell culture applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Gibco™ HBSS, no calcium, no magnesium, no phenol red
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Includes | No Sodium Pyruvate |
|---|---|
| Color | Clear |
| Physical Form | Liquid |
| pH | 6.7 to 7.8 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Hanks' Balanced Salt Solution (HBSS) |
| Concentration | 1X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 270 to 295 mOsm/kg |
| Sterility | Sterile |
| Solution Type | pH Buffer, Storage Solution and Cleaning Solution |
| Product Line | Gibco |
Gibco™ HBSS (10X), no calcium, no magnesium, no phenol red
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Gibco™ HBSS, calcium, magnesium, no phenol red
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Includes | No Sodium Pyruvate |
|---|---|
| Physical Form | Liquid |
| pH | 6.7 to 7.8 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Hanks' Balanced Salt Solution (HBSS) |
| Concentration | 1X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 270 to 305 mOsm/kg |
| Sterility | Sterile |
| Solution Type | pH Buffer, Storage Solution and Cleaning Solution |
| Product Line | Gibco |
Gibco™ DPBS, calcium, magnesium, glucose, pyruvate
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Includes | Sodium Pyruvate |
|---|---|
| Physical Form | Liquid |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | DPBS |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
| Sterility | Sterile-filtered |
| Solution Type | Dulbecco's Phosphate Buffered Saline |
| Product Line | Gibco |
Gibco™ DPBS, powder, calcium, magnesium
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Includes | No Sodium Pyruvate |
|---|---|
| Physical Form | Powder |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | DPBS |
| For Use With (Application) | Mammalian Cell Culture |
| Solution Type | Dulbecco's Phosphate Buffered Saline |
| Product Line | Gibco |
Corning™ HEPES, Powder
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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An alternative to bicarbonate for buffering
| Packaging | Plastic Bottle |
|---|---|
| Physical Form | Powder |
| Recommended Storage | 15° to 30°C |
| Sterility | Sterile |
Cell Culture Tris-Buffered Saline (10X), Corning™
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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With low endotoxin content