Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Gibco™ DPBS, no calcium, no magnesium
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Physical Form | Liquid |
|---|---|
| pH | 7.0 to 7.3 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | DPBS |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 270 - 300 mOsm/kg |
| Sterility | Sterile-filtered |
| Solution Type | Dulbecco's Phosphate Buffered Saline |
Gibco™ HBSS (10X), no calcium, no magnesium, no phenol red
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific Chemicals TAPSO, for biochemistry
CAS: 68399-81-5 Molecular Formula: C7H17NO7S Molecular Weight (g/mol): 259.273 MDL Number: MFCD00038352 InChI Key: RZQXOGQSPBYUKH-UHFFFAOYSA-N Synonym: 3-(N-Tris-(hydroxymethyl)methylamino)-2-hydroxypropanesulfonic acid PubChem CID: 109334 IUPAC Name: 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]-2-hydroxypropane-1-sulfonic acid SMILES: C(C(CS(=O)(=O)O)O)NC(CO)(CO)CO
| PubChem CID | 109334 |
|---|---|
| CAS | 68399-81-5 |
| Molecular Weight (g/mol) | 259.273 |
| MDL Number | MFCD00038352 |
| SMILES | C(C(CS(=O)(=O)O)O)NC(CO)(CO)CO |
| Synonym | 3-(N-Tris-(hydroxymethyl)methylamino)-2-hydroxypropanesulfonic acid |
| IUPAC Name | 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]-2-hydroxypropane-1-sulfonic acid |
| InChI Key | RZQXOGQSPBYUKH-UHFFFAOYSA-N |
| Molecular Formula | C7H17NO7S |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 77-86-1,7732-18-5,60-00-4,10043-35-3 Synonym: TBE
| CAS | 77-86-1,7732-18-5,60-00-4,10043-35-3 |
|---|---|
| Synonym | TBE |
Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X)
Use with Novex Tris-Glycine Gels
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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TE Buffer, 1X Solution pH 8.0, Low EDTA, Molecular Biology Grade
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
| PubChem CID | 93573 |
|---|---|
| CAS | 1185-53-1 |
| Molecular Weight (g/mol) | 157.594 |
| SMILES | C(C(CO)(CO)N)O.Cl |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
| InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| Molecular Formula | C4H12ClNO3 |
Tris-HCl, 1M Solution, pH 8.0, Molecular Biology Grade, Ultrapure
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
| PubChem CID | 93573 |
|---|---|
| CAS | 1185-53-1 |
| Molecular Weight (g/mol) | 157.594 |
| SMILES | C(C(CO)(CO)N)O.Cl |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
| InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| Molecular Formula | C4H12ClNO3 |
Invitrogen™ Tris (1 M), pH 7.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Formulation | 1,3-Propanediol, 2-Amino-2-(Hydroxymethyl)-,Hydrochloride |
|---|---|
| Physical Form | Liquid |
| pH | 7 |
| Grade | Molecular Biology |
| Recommended Storage | Store at room temperature. |
| Concentration | 1 M |
| Product Line | Ambion |
| Color | Undesignated |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Tris-Glycine-SDS |
| Grade | Electrophoresis |
| Identification | Pass Test |
| DNase | DNase free |
| Filtered Through | Filtered through a 0.2-micron filter. |
| ChemAlert Storage Symbol | Gray |
| Name Note | 10X Solution |
| Concentration or Composition (by Analyte or Components) | 0.25M Tris Base, 1.92M Glycine, and 1.0% (w/v) SDS. |
| CAS | 151-21-3 |
| Protease | Protease free |
| Synonym | TG-SDS |
| Purity Grade Notes | DNase-, RNase- and Protease-Free |
| Recommended Storage | RT |
| Packaging | Plastic bottle |
|---|---|
| Physical Form | Liquid |
| Recommended Storage | Ambient temperatures |
Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane, ultrapure, 99.9%
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | TRIS |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Invitrogen™ Novex™ Tris-Glycine Native Running Buffer (10X)
Use with NuPAGE Novex Tris-Acetate gels
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.4
Non-toxic to most cells and is used in biological research.
| Color | Colorless |
|---|---|
| Physical Form | Liquid |
| pH | 7.4 |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | Ambient temperatures |
| Concentration | 10 X |
Invitrogen™ Novex™ Tris-Glycine Native Sample Buffer (2X)
Ideal for native running conditions with NuPAGE Tris-Acetate gels
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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